It is generally believed that superconductivity can occur in materials irrespective of whether the charge carriers in the material are electrons or holes. Here we point out that Josephson tunneling would not occur between superconductors with charge carriers of opposite sign. Consequently, observation of Josephson tunneling between two superconductors implies that their charge carriers have the same sign. We propose that this has profound implications for the understanding of superconductivity and in particular is consistent with the theory of hole superconductivity.